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Part:BBa_J61212:Design

Designed by: Josh Kittleson   Group: Arkin Lab   (2007-01-31)


Tn5F|rbsGenR|FRT|R6K|CmR|FRT|P_T7|Tn5R||SpecR|OriTr|TnP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4548
    Illegal PstI site found at 4213
    Illegal NotI site found at 4126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 361
    Illegal BglII site found at 5048
    Illegal BamHI site found at 27
    Illegal XhoI site found at 4018
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4213
    Illegal NgoMIV site found at 2238
    Illegal NgoMIV site found at 3066
    Illegal NgoMIV site found at 3076
    Illegal NgoMIV site found at 3401
    Illegal AgeI site found at 4536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4680
    Illegal BsaI.rc site found at 4501
    Illegal SapI site found at 2968
    Illegal SapI site found at 3637
    Illegal SapI.rc site found at 3949


Design Notes

This is not actually a composite part on a plasmid, but rather a plasmid formed by cutting the two composite parts out with Eco and Spe, and pasting them together into a new transposon-bearing plasmid. This plasmid is not stable in cells, so any transformed cells should be used within a few days.

Source

see composite parts.

References